Growth factors from conditioned cell culture media
Rather than relying on recombinant growth factors, cultivated meat companies could use conditioned media from animal cells producing high levels of these molecules.
Seafood-relevant
Post-harvest processes and end product characterization for cultivated meat
A number of cellular processes occurring after slaughter are known to affect the quality and sensory properties of conventional meat. Cultivated meat will offer unprecedented control over these parameters and therefore over the quality of the final product, but it is critical to understand exactly how post-harvest processes for cultivated meat can or should differ from post-slaughter processes in conventional meat. This research can enable subsequent innovations in bioprocess design, media formulation, cell line development, or harvesting techniques to confer consistently high levels of meat quality from cultivated meat processes.
Establishment of cell line repositories and standardized isolation protocols
Development of humanely-sourced and thoroughly documented and characterized cell lines from a variety of common food species—together with a mechanism for licensing and distributing these lines to researchers and companies—will remove a key barrier to entry into the field of cultivated meat. In addition, development of open-access, standardized protocols for performing cell isolation from a variety of source tissues and establishing robust cell lines will streamline the processes for those who do end up needing to perform their own isolation and cell line establishment.
Cultivated meat co-product valorization
Animal cell metabolism within cultivators can produce useful co-product side streams that provide monetary value to the manufacturer while creating a novel source of inputs for other industries. Potential side streams should be identified and analyzed for their utility and economic viability, in addition to developing methods for efficient side stream capture.
Open-access formulations & optimization methods for cell culture media and growth factor cocktails
The availability of more open-access formulations will provide a foundation to enable both academic researchers and startup companies to develop their own customized formulations with far less effort and cost.
Developing assays for meat-specific cell traits
Research to align on the appropriate assays would introduce standardization that can accelerate R&D efforts.
Understand animal and cellular efficiency, yield, and input
Because cultivated meat replicates the fundamental biology of the source animal, animal-level data may be informative for predicting cellular behavior in culture.
Developing rigorous demand forecasts
Demand forecasts impact investments in R&D, infrastructure, personnel, and partnerships that will be necessary to participate in and accelerate the alternative protein sector.
Targeted alternative protein industry events & workshops
Targeted events enable greater opportunities for meaningful participation and communication between participants. They may also be especially useful for addressing specific subject matter areas or convening stakeholders with expertise in particular aspects of the alternative protein value chain to channel concerted brainstorming efforts toward actionable solutions in the form of active workshops rather than traditional conferences.
Uptake and biosynthesis of fat in cultivated meat cells
To recapitulate meat’s fat profile, research is required to determine which lipids muscle and fat cells can produce efficiently—and from which precursors—and which lipids they can absorb directly from the culture media. Understanding the effects of specific fats on organoleptic properties will help to focus these efforts.
