Uptake and biosynthesis of fat in cultivated meat cells

Current challenge

Creating cultivated meat that tastes very similar to or better than animal meat is one of the most important goals for the cultivated meat industry. A major part of this challenge is making sure that the cell culture media is supplying the key flavor components that the animal usually intakes through their diet. Identifying and understanding how lipids must be added to cell culture media in order to produce the correct level of fatty, savory, and oily mouthfeel is an important part of replicating the organoleptic experience of animal meat products. Determining that specific types of fat have negligible sensory effects would enable cultivated meat companies to focus on identifying and utilizing the most appropriate fats to create the optimal sensory experience. The results of this work would also help inform the fat solutions being created for plant-based and fermentation-based meat products.

Proposed solution

1. Conduct research to determine which fats are key contributors to taste and mouthfeel in animal-based meat.

2. For sensorily significant fats, identify which can be biosynthesized by fat and muscle cells in vitro and which need to be absorbed from the culture media.

3. Of the fats that require external sources, identify which are included in current cell culture media options and their levels of uptake by cultivated meat-relevant cell types under relevant culture conditions.

4. For fats that must be added exogenously but are not currently available in culture media formulations, identify or develop scalable, economically viable sources of these fats. These sources could be plant crops or microbial strains, with the potential need to leverage engineering approaches to develop the desired biosynthetic pathways.

It is also worth noting that the fats within animal meat evolved to serve the survival needs of the animal, not necessarily to optimize flavor for their predators. Thus, it is conceivable that fats can be identified that not only recapitulate the sensory profile of animal fats but that actually improve upon it. To take this research one step further, one could additionally identify fats not occurring in animal meat that, when added to 

plant-based or cultivated meat, improve the organoleptic properties of the meat.

Anticipated impact

This research will present multiple benefits: 

1. Fats that are critical to the mouthfeel and flavor of meat will be identified, and the specific fats that cannot be biosynthesized by cultivated meat cells can then be sourced elsewhere and added to cell culture media formulations, more effectively recapitulating an animal meat fat profile and improving the taste of the cultivated meat. 

2. The fats that are determined to have minimal to no perceived effect on meat’s sensory attributes or meaningful nutritional benefits can be omitted from the bioprocess. This provides the producer with a large amount of flexibility, because instead of focusing on perfectly recapitulating animal meat’s entire fat profile, they can focus on supplying more inexpensive, healthier, or better-tasting fats in order to reach the desired fatty mouthfeel in the end product.

This knowledge could enable cultivated meat companies to create a product that is better tasting, less expensive, and more nutritious. Knowing which specific fats confer the most organoleptic benefits would also help companies in the plant-based and fermentation sectors. Identifying unnecessary fats would enable alternative protein companies to cut costs without negatively impacting product quality, resulting in a more economically viable production process.

Related efforts

Kolanowski & Swiderski 1999. New food product containing polyunsaturated fatty acids Omega-3 EPA, DHA: sensory quality and possibility of diet supplementation.

Singh  2005. Essential fatty acids, DHA and human brain.

GFI resources

State of the Industry Report: Fermentation

This report details the commercial landscape, investments, regulatory developments, and scientific progress in the fermentation for alternative proteins industry.

State of the Industry Report: Cultivated meat

This report details the commercial landscape, investments, regulatory developments, and scientific progress in the cultivated meat and seafood industry.