Cell line development

Solutions Database

Mapping the secretome of animal myoblasts, adipocytes, and other cells used in cultivated meat

Stem cells secrete a variety of signaling factors that can influence the behavior of surrounding cells, known as paracrine signals. In high-density bioprocesses, these secreted factors can accumulate to concentrations that can dramatically influence productivity and behavior of neighboring cells. By mapping the secretome of animal myoblasts, adipocytes, and other stem cells used for cultivated meat, a better understanding of which factors influence proliferation, differentiation, and other cellular traits can be obtained. Mapping efforts will inform how to best leverage this knowledge to improve cultivated meat production.

1. Academics
2. Cell line development
3. Cultivated
4. Production
5. R&D
6. Research
7. Science
8. Seafood-relevant

Solutions Database

“Rainbow roll 2.0” showcase for cultivated seafood

Given the nascent market for cultivated meats, especially for cultivated seafood, a “Rainbow Roll 2.0” product could be an attractive market-entry commercial product.

1. 0 - Complete whitespace
2. Bioprocess design
3. Cell line development
4. Commercial
5. Cultivated
6. End Products
7. Industry
8. Industry
9. Scaffolding
10. Seafood
11. Startups

Solutions Database

Species-specific research toolkits for cultivated meat-relevant species

Coordinated efforts to develop standardized, comprehensive research toolkits of meat-relevant species would exponentially accelerate cultivated meat research.

1. Academics
2. Cell culture media
3. Cell line development
4. Commercial
5. Compliance
6. Cultivated
7. Ecosystem
8. End product formulation & manufacturing
9. GFI
10. Industry
11. NGO's
12. R&D
13. Research
14. Science
15. Seafood-relevant
16. Startups

Solutions Database

Establishment of cell line repositories and standardized isolation protocols

Development of humanely-sourced and thoroughly documented and characterized cell lines from a variety of common food species—together with a mechanism for licensing and distributing these lines to researchers and companies—will remove a key barrier to entry into the field of cultivated meat. In addition, development of open-access, standardized protocols for performing cell isolation from a variety of source tissues and establishing robust cell lines will streamline the processes for those who do end up needing to perform their own isolation and cell line establishment.

1. Academics
2. Cell line development
3. Commercial
4. Cultivated
5. GFI
6. Industry
7. R&D
8. Raw Materials, Ingredients, & Inputs
9. Research
10. Seafood-relevant
11. Startups

Solutions Database

Developing assays for meat-specific cell traits

Research to align on the appropriate assays would introduce standardization that can accelerate R&D efforts.

1. Academics
2. Cell line development
3. Commercial
4. Cultivated
5. Industry
6. Production
7. R&D
8. Research
9. Seafood-relevant
10. Startups

Solutions Database

Understand animal and cellular efficiency, yield, and input

Because cultivated meat replicates the fundamental biology of the source animal, animal-level data may be informative for predicting cellular behavior in culture.

1. Academics
2. Cell culture media
3. Cell line development
4. Cultivated
5. GFI
6. R&D
7. Research
8. Science
9. Seafood-relevant
10. Startups

Solutions Database

Uptake and biosynthesis of fat in cultivated meat cells

To recapitulate meat’s fat profile, research is required to determine which lipids muscle and fat cells can produce efficiently—and from which precursors—and which lipids they can absorb directly from the culture media. Understanding the effects of specific fats on organoleptic properties will help to focus these efforts.

1. Academics
2. Cell culture media
3. Cell line development
4. Cultivated
5. End product formulation & manufacturing
6. Industry
7. R&D
8. Research
9. Science
10. Seafood-relevant
11. Startups