The Science of Alt Protein: Glycoengineering fungal cell factories — Implications for recombinant protein production

Event description

Filamentous fungi are widely used for recombinant protein production due to their high secretion capacity and GRAS status. However, fungal N-glycosylation pathways produce high-mannose structures that differ from mammalian complex glycans, potentially affecting protein function, stability, and immunogenicity.

This presentation from André Damasio, Ph.D., will discuss glycoengineering strategies for fungal cell factories producing recombinant proteins. Using Aspergillus nidulans as a model system, Dr. Damasio explores approaches ranging from glycoproteome mapping and site-specific engineering to pathway-level rewiring via CRISPR-Cas9.

The work addresses the fundamental challenge that fungal high-mannose glycans differ from mammalian complex structures, potentially affecting protein function. Join us to learn how using advanced gene-editing tools to reprogram fungi opens new possibilities for recombinant protein production.